Rapid functional exhaustion and deletion of CTL following immunization with recombinant adenovirus.

Recombinant Adenovirus CTL following Immunization with Rapid Functional Exhaustion and Deletion of

Long-lived cytotoxic T lymphocyte memory in mucosal tissues after mucosal but not systemic immunization

The induction and maintenance of long-term CTL memory at mucosal surfaces may be a critical component of protection against mucosal pathogens and is one goal towards development of effective mucosal vaccines. In these studies we have functionally evaluated short and long-term CTL memory in systemic and respiratory or genital-associated lymphoid tissues following mucosal or systemic routes of im...

Anti-gamma interferon antibodies enhance the immunogenicity of recombinant adenovirus vectors.

Vaccination for eliciting antigen-specific memory CD8(+) T cells may be facilitated by manipulating the pleiotropic effects of gamma interferon (IFN-γ). We assessed strategies for modulating the contribution of IFN-γ during the development of antigen-specific cytotoxic T lymphocyte (CTL) populations. We first showed that recombinant IFN-γ suppressed antigen expression in vitro from a recombinan...

Influence of E1-Deleted Recombinant Adenoviruses on B7.1 and IL-2 Expression in C1498 Cells

Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1Δ) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells ...

CTLs from the peripheral blood mononuclear cells from a cervi cal cancer patient following immunization with a recombinant vaccinia using in vitro restimulation with adenovirus recombinants expressing HPV16 or HPV1S E6/E7 fusion

Human papillomaviruses (HIPVs), especially types 16 and 18, are strongly associated with the development of cervical intraepithelial neo. plasia(CIN)and Invasivecarcinoma.The HPV E6 and E7 proteinsare expressed constitutively in the majority of CIN lesions and carcinomas. Therefore, they are targets for the inunune response against HPV and candidates for active immunotherapy. We have previously...